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KMID : 0545119990090030340
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Journal of Microbiology and Biotechnology
1999 Volume.9 No. 3 p.340 ~ p.345
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Novel SSF Process for Ethanol Production from Microcrystalline Cellulose Using the δ-Integrated Recombinant Yeast,Saccharomyces cerevisiae L2612δGC
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CHO KWANG-MYUNG
YOO YOUNG-JE
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Abstract
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A novel simultaneous saccharification and fermentation (SSF) process from the microcrystalline cellulose to ethanol was developed by using δ-integrated recombinant cellulolytic Saccharomyces cerevisiae L2612δGC, which can utilize cellulose as carbon and energy sources. The optimum amount of enzymes needed for the efficient conversion of cellulose to ethanol at 30℃ was determined with commercial cellulolytic enzymes. By fed-batch cultivation, the heterologous cellulolytic enzymes were accumulated up to 42.67% of the total cellulase and 29% of the β-glucosidase needed for the efficient SSF process. When this δ-integrated recombinant yeast was applied to the successive SSF step for ethanol production, 20.35g/l of ethanol was produced after 12h from 50g/l of microcrystalline cellulose. By using this novel SSF process, a considerable amount of commercial enzymes was reduced.
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